Can someone explain the AGID assay to me?
Answers:
See links to support notion:
It appears that AGID can be run from blood, and is an indicator of exposure due to the presence of antibodies. It does not indicate specific types of influenza.
"Rapid diagnosis can be made by the detection of viral antigen in tissue impress smears using immunofluorescence, or by antigen detection enzyme-linked immunosorbent assay (ELISA) on tissue homogenates. Pancreas and kidney are the organs in which antigen is most habitually demonstrable." (link)
This suggests AGID is easier/ faster and perhaps more cost efective for trialling large numbers of specimins beside further testing just on those testing positive.
The AGID testing is an Agarose Gel Immunodiffusion assay. Basically, you put an antigen of interest (a virus) and a serum sample from an animal into next to wells of an agar plate. The antigen/antibody complexes that form (if any) should form a flash between the two gels, primarily solid precipitates in the agar. As far as I can share, the specificity and sensitivity of this test (as you would expect, base on eye detection) is really low. ELISA analysis with an avian influenza virus antigen would be more accurate, if it is available.
Related Questions and Answers
How is approaching mortal an anaesthesiologists?
I know the length of schooling, but what do they mean by residency? What do you do on a daily proof at work? Ddo you find the job repetitive? Are anaesthesiologists asked to administer lethal injection? And do you think its pretty cool that you can put someone to sleep? its okay Well.....
Do you know what this is?
The chain of infection consists of a(n) A. infectious agent B. reservoir host. C. mode of transmission. D.portal of entry. E. All of the above The method that most hospitals use to reduce the risk of disease nouns by means of moist body substances is A. Standard Precautions B. Universal Precautions C. personal protective equipment....