Prolonged tourniquet pressure during venipuncture may spuriously increase total calcium?

could you please tell me the basis?
Answers:
A tourniquet is consistently used to obstruct the return of venous blood to the heart, and it should not be moved out in position for more than one minute, because it cause the moving of fluid and low molecular compounds from the intravasal space to the interstitium. Fluid migration is due to the increased filtration pressure, similar to that in shifting posture from lying to standing. Macromolecules, compounds bound to protein and blood cells cannot follow this, hence their concentration rises. The plasma/serum analyte concentrations and coagulation factors are not influenced by the constriction time of one minute beside successive releases of the tourniquet. Prolonged tourniquet application entails change in the concentration of lots analytes, e.g., total protein, calcium, alanine aminotransferase, aspartate aminotransferase, creatine kinase, bilirubin, and potassium
Prolonged pressure also increases the likelihood of hemolyzing the red cell thereby releasing inter cellular ions into the blood draw (potassium & calcium being two major ones)


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