At what temperature would you incubate bacterial cultures of clinical specimen? Why?
Answers:
37C for the vast majority. for C. Diff cultures 42C. cultures are not kept at room temp.
the bacteria that are clinically significant grow best at body temp. the lab is using optimal conditions for the best recovery. mimicking the conditions that the germs normally thrive.
Well, it is true that bacterial cultures (in general) grow optimally at room temperature - 25°C.
However, clinical specimens are incubated at body heat - 37°C. This should make sence considering you are looking for bacteria that is infecting someone - to be exact, able to thrive and multiply in the environment the human body provides. Therefore, to observe as copious of the potential pathogens as possible, wouldn't it be best to provide an environment as close as possible to the human body? This is not temperature-exclusive. Many hospital labs use blood agar (media for culturing bacteria) to produce pure cultures for a positive ID of a specimen. So in addition to controlling temperature, the type of nutrients available to the germs is important to match as well.
Hope that help.
about 25 degrees centigrade thats is the optumum temp for a culture of germs usually.
I visited a biology lab in a hospital lately, and the way they do it is to keep the specimens at body temperature - 98.6 F, 36 C - as okay as at room temperature. Then they Gram stain samples them to identify the bacteria lower than the microscope. It also depends on the type of bacteria, as well as whether or not they grow in air-filled environments or within a vacuum. Hope this helps! Good luck!
not all bacteria have like incubation temperature , but most do use the 36-37C range as it mimics human body temperature. so it solely makes sense to grow them in that temp.. it would be useless otherwise.
36C and room temp. The most relevant species grow at body and room temp.
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